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![]() Just please.” He kisses me deeply and flips me onto my stomach. He starts to move down, but I pull him back up, He unclasps my bra and then removes my pants as well. He sits up and unbuttons them before pulling them and his boxers off. I moaned again and tried to pry him out of the confined restraints of his pants. “You don’t know how long I’ve waited for this,” he whispered. His mouth goes to my neck and he starts kissing and biting. I take off his shirt and he flips us over. He kisses up my torso before reconnecting our lips. We stay like this for a short time until he’s taking off my shirt. I push him back and he pulls me on top of him. His lips are soft, but sticky from the weed. He meets me half way when I grab his face in one hand and lean in to kiss him. He looks confused at first, but then gets comfortable. I put the joint out on the nightstand and go to lay down with him. When I turn back to grab it a third, I make sure to brush his fingers with mine. We do this two more times before the high really sets in. I take my hits before turning back to him. I walk over to him and grab it and take a seat at the foot of his bed. He sparks up a joint and takes a couple of hits before offering it to me. I stop at the second doorframe, feeling a little weird that I masturbated to my best friend last night. He saunters over to his bed and lays down. I take him in until I realize I’m staring. I knock on his bedroom door and he answers. I’ve haven’t had this feeling about him for long, but after last night he’s all I want. The next day, I get a text from Elliot asking me to come over and I take a while to respond, but agree to go. “Fuck,” I sigh and bury my face in my pillow. I press it against until I’m moaning his name. I reach as far as I can and feel that spongy organ. I insert two fingers and pump them in and out. The memory of his pointed tongue going in and out, making sure to hit every wall created by my palm. ![]() My other hand made its way to my throbbing clit and started rubbing. The way he swirled it around as if he were going in small circles with his cock. I looked at my hand and I could still feel his tongue. That night in bed, all I could think about was what happened at Elliot’s. I chuckle at him and we just looked at each other for a moment before Jules and Rue popped in. “Not necessarily right now, but I do like pushing those boundaries.” Plus, seeing a pussy drip with my cum makes me feral.” He shrugged, "I mean I guess playing with the thought of my girl carrying around my kid makes me hard. “So, basically you have a breeding kink?” Eating your own cum out of a pussy? Not a lot of people do that.” I think anal is a little more mainstream now. “What do you mean ew? That’s one of the most personal things you can do with your partner.” “Oh, Elliot! Ew!” He leaned back and laughed dramatically. He takes a second and then laps up his mess from my hand and I pull away again. He collects it and pushed it into my palm. “See, that’s a dirty cunt.” He turns my hand back and starts smearing around what has seeped out. He turns my hand to me and I see the white substance leaking out of the hole in my fist. He looked at me and smirked before letting spit leak into my hand. He continues to plunge his tongue before pulling away. C'mon.” I look him in his eyes before slowly giving my hand back to him. “Y/n, this wouldn’t be realistic if the pussy wasn’t wet. “Ugh! What are you doing?” I asked inspecting my hand. He starts to lick the makeshift pussy and I instinctively pull my hand away. His large palms close mine into a soft fist, “say this is your pussy, right?” “Think about it, Y/n,” he dives on the bed and takes my hand. I’m going to give you pleasure so good you’ll forget your name… You’re going to think my name is Yes, God Yes, or Fuck Yes. WARNINGS: PURE SMUT, Language, (f)masturbation, creampie, breeding kink. The locking system is a useful feature that protects and secures the data.This security system also controls by encrypting valuable data.USB encryption security system restricts the data flow.USB encryption system helps to protect sensitive data on external devices.The following are the reasons why we need a USB encryption security system: Why do We Need a USB Encryption Security System? The most common applications they make use of USB encryption are: There are two types of USB encryptions available they are: Encryption is a method that restricts data access either by software or hardware installed on the hard drive with key locked. USB is a flash drive encryption device that ensures your private data is protected as well as secured. Bitlocker A robust and compliance security software that supports windows operations and is a highly lightweight handy encryption software, easy to carry and use from anywhere.Ī Quick Introduction to USB Encryption Software.KakaSoft USB Security A run-of-the-mill type of software that offers minimal features, however, it is a fully self-contained software on the USB flash drives, so it’s straightforward to carry and can be used anywhere.It comes in the form of a handy when you want to encrypt folders or USB drives. DriveCrypt A one-stop solution that guarantees the security of the files.Gilisoft USB Stick Encryption Pretty and funky user interface features, a very easy-to-use encryption system.Rohos Disk Encryption Packs good features, and the core functionalities of this encryption system are the creation of encrypted volumes.EncryptStick A type of encryption system that allows users to encrypt USB drives and also helps to create virtual folders or files protected with a password. ![]()
![]() ・If you cancel your subscription before the renewal date, you will still be able to enjoy the benefits until the end of your membership period. Only first-time subscribers are eligible for the free trial. Your Apple account will be charged when making a purchase. Gain access to VIP-exclusive items in the shop. You will be billed for the automatic renewal within 24 hours of the subscription’s expiration date. If you do not cancel the paid VIP membership or free trial at least 24 hours before the subscription’s expiration date, your subscription will automatically be renewed. Your subscription will automatically be renewed 1 month after the start date. Collect cards, level them up, and win the battles! ![]() Choose your own team and see the different chibi characters appear on-screen as you listen to the music that varies each stage. Maybe even a kiss from an ikemen demon awaits?Įvil demons in the Devildom are after your soul! With the help from the 7 brothers, you can defeat them in an exciting card battle. There is only 1 rule you have to remember to survive.Ĭhoose how you wish to enjoy your life: Will you whip the demon brothers into shape, or pursue a new romance? Awaiting you are 7 demon brothers and a mountain of tasks.?! You have been selected to become an exchange student at RAD, a school for demons. There is no gender selected for the main character, so anyone can play.īecome the master who brings the handsome demon brothers down to their knees and enjoy life at the Academy in the Devildom! The way you interact with the characters will affect your relationship with them. ![]() There is an intimacy level system in the game. You regularly get text messages and phone calls from the charming characters. "Obey Me!" is a simulation game that makes the characters a part of your everyday life. What if the characters could come by your side, laugh and cry together with you, and become your friend? ![]() SEN eventually escapes to an area reserved for the monks of OMM, where a lone monk notices that SEN has no identification badge. Chased by the police robots, THX and SRT are trapped in a Control Center, from which THX learns that LUH has been "consumed", and her name has been reassigned to fetus 66691 in a growth chamber. During the escape, THX and SRT are separated from SEN. Most of the prisoners seem uninterested in escape, but eventually THX and SEN find an exit they are later joined by hologram SRT 5752, who starred in the holo-broadcasts. THX enjoys a brief reunion with LUH, disrupted shortly after she reveals her pregnancy.Īt THX's trial, THX is sentenced to prison, alongside SEN. After the release of the mind lock, THX makes the necessary correction to that work phase. Without drugs in his system, THX falters during a critical and hazardous phase of his job, and a control center engages a "mind lock" on THX which raises the level of danger. THX later is confronted by SEN, who arranges THX as his new roommate, but THX files a complaint against SEN for the illegal housing mate change. The drug substitution also leads to LUH and THX becoming involved romantically, resulting in the two engaging in intercourse. LUH secretly substitutes pills in her possession for THX's medications, whereupon THX eventually suffers physical discomfort (vomiting) along with mental/emotional changes (confusion). Work hard, increase production, prevent accidents and be happy".Īt home, THX takes his drugs, and watches holo-broadcasts while engaging with a masturbatory device. A soothing voice greets THX, and OMM ends every confession with a parting salutation: "You are a true believer, blessings of the State, blessings of the masses. ![]() On the way home, he stops at a confession booth in a row of many, and mumbles prayers about "party" and "masses", under the portrait of "OMM 0910". At the beginning of the story, THX leaves the job while the loudspeakers urge the workers to "increase safety", and congratulate them for only losing 195 workers in the last period, to the competing factory's 242. LUH has a male roommate, THX 1138, who works in a factory producing android police officers. Instead of names, people have designations with three arbitrary letters (referred to as the "prefix") and four digits, shown on an identity badge worn at all times.Īt their jobs in central video CCTV control centers, SEN 5241 and LUH 3417 keep surveillance on the city. Everyone is clad in identical uniforms and has shaven heads to emphasize equality, except the police androids (who wear black) and robed monks. Emotions, coitus, and the concept of family are taboo. In the 25th century, sexual intercourse and reproduction are prohibited, whereas use of mind-altering drugs is mandatory to enforce compliance among the citizens and to ensure their ability to conduct dangerous and demanding tasks. ![]() The computers automatically quarantine suspicious files, meaning that they can’t access critical system software or change the computer’s core functions. In general, the design of Macs limits damage from malware. If the tool identifies malware, it will notify you and remove the dangerous files. Since threats are always changing, the Mac Malware Removal Tool also scans your computer regularly to check that nothing sneaks past XProtect. If XProtect detects malware on a file or application, it notifies the user and blocks the download. XProtect scans all applications and files for viruses and malware using a database of threats that Apple updates daily. Since 2009, Apple has included XProtect, Apple’s proprietary antivirus software, on all Macs. Avast Antivirus – Mac is Protected Do Macs Have Built-in Antivirus Software? Is Apple doing anything about that? Do Macs have built-in antivirus software? Do you need a third-party antivirus app for your Mac? We answer all those in this guide. In fact, Malwarebytes, an antivirus company, saw a 400-percent increase in Mac malware in 2019 compared to 2018. Macs have grown in popularity, and cybercriminals saw that as an opportunity to target more people, focusing their efforts in creating malware for Apple computers. While that may have been true 20 years ago, it no longer applies today. There’s even a myth that Macs are safe from malware, mainly because most viruses are designed for Windows. A PC malware designed to exploit a Windows vulnerability, for example, does no harm on a Mac computer. Here’s one thing many people don’t know about computer viruses: Many of them only attack specific operating systems.
![]() With just a few essential techniques, you'll be quickly on your way to making high-quality effects with Adobe After Effects.įirst and foremost, start by exploring the After Effects workspace. As daunting as it looks, After Effects is surprisingly user friendly and can be mastered with relative ease. Essential Techniques for Navigating the Adobe After EffectsĪdobe After Effects is an in-depth and powerful software program that can help create specialized graphic effects, and produce animation for both film and television. Knowing these tools will help you make the most from your After Effects projects. After Effects also features a 3D Camera Tracker and 3D system to turn any shot into stereoscopic 3D.These include the Keyframe Tool which is used to set the timing of animations and the Graph Editor which is used to customize the motion curves for more precise animations. ![]() Finally, After Effects also includes various tools for adding animation to your projects.In addition, the Pen Tool allows you to create precise paths by drawing bezier curves and lines on top of footage.The Puppet Tool is an advanced tool used to create complex distortions while the Roto Brush is used to easily select and mask objects in footage.This allows you to move and modify layers within the composition and adjust attributes such as position, opacity, masking, and blending modes. The most commonly used After Effects tool is the Layer Tool.To get the most out of After Effects, it’s important to have an understanding of the various tools available. After Effects allows you create high-quality 2D and 3D graphics, natural looking animations, and sophisticated visual effects that are used in film, television and digital media. Understanding the tools of Adobe After Effects is the first step to mastering this powerful animation and compositing application. Get creative, unleash your imagination and bring your dreams to life! Understanding the Tools of Adobe After Effects There is no better way to get started than by learning the basics of Adobe After Effects. With its limitless possibilities and intuitive operations, After Effects has to be experienced to be fully appreciated. Every visual effect can be created from scratch, allowing users to adjust the amount of detail and the speed of the motion graphics to suit their individual needs. Whether creating titles, graphics, or animations, Adobe After Effects can help to bring any project to life. The built-in Animation Presets speed up the process while also offering after effects artists creative control over their finished product. After Effects offers an extensive range of tools and effects, including 3D rendering, rotoscoping, and color correction, which can quickly and easily be manipulated with Effects Controls and processes. However, with a little practice and dedication, users will be comfortable and proficient in no time. The main interface of After Effects can be intimidating at first glance. With After Effects, users can add and combine visuals of almost any kind to produce breathtaking scenes, eye-catching animations, and outstanding VFX. The software is used extensively in the post-production industry, allowing studios and single individuals to generate amazing work for film, television, and web. Introduction to Adobe After EffectsĪdobe After Effects is a powerful motion graphics and animation software that enables anyone to create high resolution, sophisticated and professional visual effects. Follow MedMehrez for all the latest and greatest in After Effects and video editing tutorials. Whether you're a beginner or an expert, MDMZ is a great place to get started or brush up on your skills. ![]() Led by Mohamed Mehrez, a talented filmmaker, it features a range of tutorials in Adobe After Effects and other visual tools like motion graphics and VFX. المملكة العربية السعوديةThe MDMZ YouTube channel is an amazing resource for all types of video editing and visual effects enthusiasts. ![]() Česká republikaĬyprus - EnglishĭanmarkĭeutschlandĮestiĮspañaįrance ประเทศไทยĮurope, Middle East and AfricaĪfrica - Englishīelgië - Nederlandsīelgique - Françaisīelgium - English Southeast Asia (Includes Indonesia, Malaysia, Philippines, Singapore, Thailand, and Vietnam) - English ĪmericasīrasilĬanada - EnglishĬanada - Français.Selecting a region changes the language and/or content on. ![]() ![]() Sorry if this sounds noob-ish, but I really don't know this. It's a nice tutorial, but I'm still a bit lost on (at least) three things: Can you or anyone else help me out at this point? Is this a matter of cropping the black border, resizing and fitting it in that template at that point? by selecting the category tabs below Save time. ![]() This could be normal - it IS called wii banner player, after all - but would you know how to make these files?ģ) I take it you need to play the banner for the boottxt file and unpacking the icon.bin for the icontex file.but neither have the correct format (and the first has this huge black bottom). You searched for 'Dokapon Kingdom wii' in All Sections: 3 Results Found Switch between ROMs, Emulators, Music, Scans, etc. The cool thing is that it grabs and shows all bnr files in the folder (if you click on one, it'll play that one), but it doesn't show gamecube opening.bnr's. I got this from the internet, but could only get this to work by dragging an opening.bnr file onto it. tpl files you want to convert.Ģ) could be my windows 10 64 bit OS, but wii banner player wanted openal32.dll. Namely that you have to run U8Mii two times (the first time on opening.bnr, and the second time on icon.bin) to get the. GczA program that can automatically replace the files in a Dokapon Kingdom iso/wbfs. Click to expand.It's a nice tutorial, but I'm still a bit lost on (at least) three things:ġ) there is a part missing just before the "now turn the tpl into png" part. Open ISO file Complete solution for Wii gamesConvert ISO to WBFS. ![]() There is increasing evidence that LTA4H is overexpressed in many malignant cancers, which promotes cancer cell proliferation. However, more study is necessary to fully understand the specific molecular mechanism of LTA4H in laryngeal carcinoma. More importantly, studies have demonstrated that increased LTA4H expression in LSCC is associated with a poor prognosis, and knockdown of LTA4H successfully suppresses the growth, invasion and migration of laryngeal carcinoma cells ( Gao et al., 2019 Peyvandi et al., 2018). GO function was significantly enriched in RNA processing and respiratory electron transport chains, and LTA4H was one of the up-regulated proteins ( Peyvandi et al., 2018). A recent study screened 275 differential proteins associated with laryngeal cancer through PPI (protein-interaction) network analysis. The results showed that LTA4H was significantly up-regulated in LSCC tissues than in normal tissue. (2019) detected LTA4H expression in LSCC and normal tissue by qPCR. ![]() In recent years, researchers have found that many genes, miRNAs and lncRNAs are key factors of LSCC ( Zhang et al., 2016). Genes, miRNAs and lncRNAs all play key roles in tumor genesis and development. ![]() Hence, to better identify biomarkers and explore effective new therapeutic strategies, it is essential to reveal the LSCC’s carcinogenic mechanism. Studies have revealed lincRNA HOTAIR is highly expressed in LSCC and promotes methylation of PTEN ( Li et al., 2013). It has also been reported that the ANXA1 interaction with FPR2/ALX promote proliferation and metastasis of LSCC ( Gastardelo et al., 2014). Studies have shown that the prognosis of patients with LSCC with down-regulated HLA class I antigen was worse ( Ogino et al., 2006). An increasing amount of research demonstrates that the formation and development of LSCC is related to molecular mechanisms. Due to LSCC’s uncertain molecular mechanism, over 60% of patients were already at late stages when the disease was discovered ( Steuer et al., 2017). Due to the proneness to recurrence and metastasis, the five-year overall survival rate for LSCC has been approximately 50% in recent years ( Cavaliere et al., 2021). Research shows that nearly 180,000 new cases of throat cancer and nearly 100,000 throat cancer deaths were reported worldwide in one year ( Bray et al., 2018). 95 to 98% of laryngeal cancers are squamous cell carcinomas (LSCC) ( Zuo et al., 2016). Laryngeal cancer is not only the most common malignancy of the head and neck, but also the second most common respiratory system tumor after lung cancer. Our study further sheds light on the molecular mechanism of LTA4H as a clinical therapy target for LSCC. These results suggest that LTA4H may combine with genes associated with LSCC as an RNA-binding protein to perform a cancer regulatory function. QRT-PCR validation confirmed that LTA4H specifically binds to mRNAs of carcinogenesis-associated genes, including LTBP3, ROR2, EGFR, HSP90B1, and lncRNAs represented by NEAT1. More notably, LTA4H-binding genes were significantly enriched in the mitotic cell cycle, DNA repair, RNA splicing-related pathways, and RNA metabolism pathways, which means that LTA4H has tumor-related alternative splicing regulatory functions. In the LTA4H binding peak, the frequency of the AAGG motif reported to interact with TRA2 β4 was high in both replicates. We found that LTA4H extensively binds with mRNAs/pre-mRNAs and lncRNAs. To investigate the potential role of LTA4H in LSCC, we employed the improved RNA immunoprecipitation and sequencing (iRIP-Seq) experiment to get the expression profile of LTA4H binding RNA in HeLa model cells, a cancer model cell that is frequently used in molecular mechanism research. Recent research shows that the increased expression of LTA4H in laryngeal squamous cell carcinoma (LSCC) promotes tumor proliferation, migration, and metastasis. The RNA-binding metabolic enzyme LTA4H is a novel target for cancer chemoprevention and chemotherapy. LTA4H extensively associates with mRNAs and lncRNAs indicative of its novel regulatory targets. Cite this article Ren T, Wang S, Zhang B, Zhou W, Wang C, Zhao X, Feng J. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China DOI 10.7717/peerj.14875 Published Accepted Received Academic Editor Vladimir Uversky Subject Areas Biochemistry, Cell Biology, Molecular Biology Keywords LTA4H, RNA-binding protein, lncRNA, iRIP-seq, Laryngeal tumorigenesis Copyright © 2023 Ren et al. No Correlation: there is no apparent relationship between the variables.Time spent studying and time spent on video games are negatively correlated as your time studying increases, time spent on video games decreases. Negative Correlation: as one variable increases, the other decreases.Height and shoe size are an example as one's height increases so does the shoe size. Positive Correlation: as one variable increases so does the other.There are three types of correlation: positive, negative, and none (no correlation). With scatter plots we often talk about how the variables relate to each other. Maybe his father is giving him more hours per week or more responsibilities. For example, with this dataset, it is clear that Mateo is earning more each week. Using this plot, we can see that in week 2 Mateo earned about $125, and in week 18 he earned about $165. In general, the independent variable (the variable that isn't influenced by anything) is on the x-axis, and the dependent variable (the one that is affected by the independent variable) is plotted on the y-axis. The weeks are plotted on the x-axis, and the amount of money he earned for that week is plotted on the y-axis. Here's a scatter plot of the amount of money Mateo earned each week working at his father's store: These types of plots show individual data values, as opposed to histograms and box-and-whisker plots. Also keep in mind that other factors may be involved in a cause-effect relationship.Scatter plots are an awesome way to display two-variable data (that is, data with only two variables) and make predictions based on the data. Of course this is not true!Īlways be careful what you infer from your statistical analyses. So, this must mean that the number of jars of strawberry jam sold in New York was causing an increase in the number of classical music CDs sold in Florida. The data was examined and was plottedįrom looking at the graph, it can be seen that there is a high positive correlation between these two sets of data. For the same time frame, the number of copies of a popular classical music CD sold in Florida was recorded. No correlation means that the data just doesn’t show if studying longer has any affect on Regents examination scores.Ĭheck out these graphs for visual interpretations of types of correlations:ĭuring the months of February and March, the weekly number of jars of strawberry jam sold at a local market in New York was recorded. If the plot on the graph is scattered in such a way that it does not approximate a line (it does not appear to rise or fall), there is no correlation between the sets of data. Under a negative correlation, the longer I study, the worse grade I would get on my Regents examination. If the slope of the line had been negative (falling from left to right), a negative correlation would exist since the slope of the line would have been negative. It all depends on the data being examined. There may be sets of data that show that there is NOT a positive correlation between hours studying and better Regents scores. Note: Just because this set of data showed a positive correlation does not mean that the relationship is positive for all sets of data concerning study time and Regents scores. This means that according to this set of data, the longer I study, the better grade I will get on my Regents examination. Since the slope of the line is positive, there is a positive correlation between the two sets of data. The data displayed on the graph resembles a line rising from left to right. Notice: Certain values may have more than one result, Remember when making a scatter plot, do NOT connect the dots. Given the data below, a scatter plot has been prepared to represent the data. Let’s decide if studying longer will affect Regents grades based upon a specific set of data. Scatter plots will often show at a glance whether a relationship exists between two sets of data. Statisticians and quality control technicians gather data to determine correlations (relationships) between such events. |
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